Fig. 2

Morphology and gene expression in the four GBM cell lines used in this study. a SB2b and PB1 of classical GBM subtype; JK2 of proneural subtype and WK1 of mesenchymal GBM subtype as adherent cultures on matrigel, scale bar represents 100 μM. b Expression of CALCR, CALCRL, RAMP1, RAMP2, RAMP3, CALCA, and IAPP in SB2b, PB1, WK1 or JK2 cells. Data represent mean + SEM of 3 independent experiments, performed in duplicate, relative to β-actin expression. ND (not detected in all 3 samples), 2/3 (in 2 out of the 3 samples mRNA was detected), 1/3 (in 1 out of the 3 samples mRNA was detected). c Western blot for CTR, 50 μg of membrane protein probed with anti-CTR 1H10, representative of 3 independent experiments