Fig. 2From: The garlic compound ajoene covalently binds vimentin, disrupts the vimentin network and exerts anti-metastatic activity in cancer cellsValidation of Vimentin as an Ajoene Target. (a) Immunoblot of human recombinant vimentin treated with DP (100 μM) in the absence or presence of DTT (100 mM), probed with a primary anti-dansyl and anti-vimentin (H-84) antibody. (b) Human recombinant vimentin was treated with 100 μM ZA or DP and purified by SDS-PAGE. The band excised from the gel was digested with trypsin and fragments were identified by MS/MS MALDI-TOFF mass spectrometry. The Cys-328 containing fragment qvqsltcevdalk was detected in the control and treated samples carrying a 2+ charge where m/z = [M + 2H]2+. (c) In the samples treated with ZA or DP, the predicted m/z ratio of the modified fragment was observedBack to article page