Fig. 2

Biological effects of CK2 inhibition on B-ALL cell lines. a Proliferation was determined using trypan blue staining after 72 h incubation of increasing CX-4945 concentrations and compared to control cells set to 100% (mean + SD; n ≥ 2; * p < 0.05, ** p < 0.01, *** p < 0.005 for n ≥ 3 experiments). b The WST-1 assay was used to evaluate metabolic activity after 72 h incubation of increasing concentrations of CX-4945. Three technical replicates were performed for each sample (mean + SD; n ≥ 3; * p < 0.05, ** p < 0.01, *** p < 0.005). c The effect on apoptosis was measured by Annexin V-FITC/PI following 72 h CX-4945 incubation. Early apoptotic cells (Annexin V⁺/PI⁻) are located in the lower right quadrant while late apoptotic and necrotic cells (Annexin V⁺/PI⁺) are located in the upper right part. (n ≥ 2)