Clinical impact of a cytological screening system using cyclin D1 immunostaining and genomic analysis for the diagnosis of thyroid nodules

Table 5 Comparison of genetic variants identified by target NGS in LBC and FFPE samples of thyroid tumors

Patient no	Gene	cDNA change	Amino acid change	Variant frequency (%)	Read depth	Functional consequence
Case 29 LBC	HRAS	c.81A > G		50	104	synonymous variant
Case 29 LBC	TP53	c.215G > C	p.72P/R	99.66	1468	missense variant
Case 29 FFPE	HRAS	c.81A > G		48.75	80	synonymous variant
Case 29 FFPE	TP53	c.215G > C	p.72P/R	98.86	612	missense variant
Case 30 LBC	BRAF	c.1799A > T	p.600 V/E	31.98	172	missense variant
Case 30 FFPE	BRAF	c.1799A > T	p.600 V/E	2.87	348	missense variant
Case 31 LBC	BRAF	c.1799A > T	p.600 V/E	40.46	351	missense variant
Case 31 FFPE	BRAF	c.1799A > T	p.600 V/E	25.68	183	missense variant

LBC liquid based cytology sample, FFPE formalin fixed paraffin embedded specimen

ISSN: 1471-2407