Fig. 4
From: Genome-wide CRISPR screen reveals PSMA6 to be an essential gene in pancreatic cancer cells
PSMA6 knockdown results in apoptosis and reduced spheroid formation. a Flow cytometric analysis and quantification of 7-AAD and annexin V staining in b PANC-1 cells and c Mia PaCa-2 cells 72 h post transfection with siControl (non-targeting siRNA) or siPSMA6. d Western blot of siControl and siPSMA6 samples probed with PSMA6 and tubulin antibodies after 72 h of siRNA treatment. e Quantification of the western blot with the samples normalized to tubulin expression levels. f PSMA6 levels after 500 nM doxycycline treatment in stable PANC-1 cells expressing a tet-on PSMA6 shRNA sequence (tet-on shPSMA6). g Spheroid formation at days 1 and 10 after doxycycline induction in tet-on shPSMA6 PANC-1 cells and h quantification at day 10 with a cell viability assay (CellTiter-Glo). (*P = 0.05; ***P = 0.01, ****P = 0.0001)