Fig. 5

Analysis of genomic regions of coherent up- or down-regulation in sarcoma vs. normal tissue. a 10-Mbp genomic regions showing statistically significant (p < 0.05, based on permutation test, and absolute log₂(sarcoma/skin) > 1) and coherent up- or down-regulation of the genes within the region, in sarcoma vs. normal skin tissue. Each bar corresponds to a specific 10 Mbp region in the cat genome. Bars are labeled by Felis catus chromosome and the start coordinate of the region, in Mbp (e.g., “Fc_C1:70”). Bars indicate the average log₂(sarcoma/skin) values for all genes within the indicated region. Asterisk indicates a concordance of the transcriptional analysis of the indicated region with a recurrent deletion in FISS as reported in a previous array comparative genomic hybridization study [4]. b Circos-style graphical depiction of coherently up- (red spokes) or down- (blue spokes) regulated 10 Mbp regions in sarcoma vs. normal skin (see colormap). Cat nuclear chromosomes are arranged clockwise around the circos plot from 12:00 to 5:00; human nuclear chromosomes are arranged clockwise from 5:00 to 12:00. Curved light gray arcs indicate syntenic regions of the human genome that correspond to the regions of coherent up- or down-regulation in FISS. Gray diamond denotes syntenic human genomic region that is recurrently deleted in human soft-tissue sarcoma (see Methods), according to the cBioPortal database [37]