Fig. 7

Anisomycin based p38 MAPK activation affects expression of various cytokines and MMPs: a. Treatment of the DU145 cells with 0.4 μg/ml of Anisomycin (p38 MAPK activator) led to enhanced phosphorylation of p38 MAPK compared to the mock. Activation state was observed at 2 h (Lane 1 and 2) and 6 h (Lane 3 and 4) time points. b. Treatment of the PC3 cells with 0.4 μg/ml of Anisomycin led to enhanced phosphorylation of p38 MAPK compared to the mock. Activation state was observed at 2 h (Lane 1 and 2) and 6 h (Lane 3 and 4). c. Expression of GM-CSF, MMP1 and PLGF levels were quantified in DU145 cells after 8 h of treatment with 0.4 μg/ml of Anisomycin. GM-CSF (Fold change = 18.97) and PLGF (Fold change = 2.73) were significantly up-regulated on Anisomycin treatment, no significant effect on MMP1 (Fold change = 0.67) levels were observed. d. Expression of GM-CSF, MMP1, PDGFA and CXCL1 levels were quantified in PC3 cells after 8 h of treatment with 0.4 μg/ml of Anisomycin. GM-CSF (Fold change = 82.24), MMP1 (Fold change = 2.07) and CXCL1 (Fold change = 11.98) were significantly up-regulated on Anisomycin treatment, no significant effect on PDGFA (Fold change = 1.36) levels were observed. In panel C and D, t-test (2 tailed, for unpaired samples) was used for comparison of fold change values between experimental and control group. Each experimental group had three replicates. # represents p value > 0.05, * represents p value < 0.05, ** represents p value < 0.01, *** represents p value < 0.001