Fig. 3

Numb reduces Notch signaling- and EMT-associated genes expression. a Western blot analyses shows protein levels of Numb, Notch1, RBP-Jκ and EMT-related genes (E-cadherin, N-cadherin, Snail-1, MMP-9) in mock or Numb-overexpressed SCC-9 and CAL-27 cells. GAPDH acts as internal control. b Gray level analysis of the protein levels of Numb, Notch1, RBP-Jκ and EMT-related genes (E-cadherin, N-cadherin, Snail-1, MMP-9) in mock or Numb-overexpressed SCC-9 and CAL-27 cells after western blot. The mean ± SD in the graph represents the average levels from three replicates. *p < 0.05, **p < 0.01, ***p < 0.001