Fig. 5

RBP-Jκ-depleted tongue cancer cells exhibits attenuated proliferation, invasion and migration. a Cell proliferation assay and quantification of NC (sh-NC) or RBP-Jκ-knockdown (RBP-Jκ shRNA) SCC-9 and CAL-27 cells by MTT assay at 48 h after transfection. b and c Wound healing assay and quantification shows the migration ability of NC (sh-NC) or RBP-Jκ-knockdown (RBP-Jκ shRNA) SCC-9 and CAL-27 cells. The photographies were taken at different time points of 0, 24, 48 h. Magnification × 10(5000 μm) in wound healing assay. d and e Transwell invasion assay and quantification shows the invasion ability of NC (sh-NC) or RBP-Jκ-knockdown (RBP-Jκ shRNA) SCC-9 and CAL-27 cells. The photographies were taken at 24 h. The photographies were taken at 24 h. Magnification× 50(2000 μm) in transwell assay. The data were represented as mean ± SD from 3 biological replicates (*p < 0.05; **p < 0.01)