Fig. 1
From: Oncogenic Ras is downregulated by ARHI and induces autophagy by Ras/AKT/mTOR pathway in glioblastoma
ARHI inhibits glioma cell growth in vitro. a ARHI mRNA relative expression in glioma and normal brain tissues. b ARHI expression in different grades of glioma tissues and normal brain tissues measured by western blot assays. T1-T3 grade IV; T4-T6 grade III; T7-T9 grade II; N1-N3 normal tissues. c ARHI expression in different glioma cell lines and human brain astrocyte cell line (NHA) (d) Relative ARHI mRNA expression in four different glioma cell lines. e-f Colony formation assays of LN229 and T98G cells after over-expressing ARHI contrast with the negative control. The experiments were repeated 3 times independently, and the bars represent SD. The data were normalized to the control group (*p < 0.05, **p < 0.01). g-h Fluorescence microscopy of Ki67 expression after transfection with ARHI and negative control plasmids. The scale bar represents 100 μm