Fig. 3

Reduction in the myeloid immune compartment in the absence of NK cells PEDF does not reduce hepatic metastases. a-c Excised livers from all groups were macerated for RNA extraction, cDNA synthesis and examination of mRNA expression. a Gene expression analyses of the type-1 genes Socs3 and Tnfa mRNA and the type-2 Arg1 and Tgfb mRNA using the ∆C_T Comparative method. b The pro-apoptotic Casp3 mRNA and the tumor suppressor Trp53 were examined using the ∆C_T Comparative method, as well. c Cd68 mRNA, typical of infiltrative macrophages, was examined in the bulk liver tissue. All Data represents mean ± SEM. All mRNA analyses display the fold change of target gene over endogenous control (Gapdh)