Fig. 5

Adjuvanted E7 SLP promotes antigen specific memory CD8 T cells durable T cell functionality. Mice (n = 5) were immunized s.c. on day 0 and 21 with 20 μg E7 SLP + CpG +/− o/w emulsion. Splenocytes and PBMCs were isolated on day 28. a Cells were FACS analyzed for presence of E7 dextramer⁺ CD62L- CD44^hi KLRG1^hi populations. b Mice (n = 5) were immunized s.c. on day 0 and 21 with 3 μg E7 SLP + o/w emulsion +/− CpG. Splenocytes were isolated approximately 3 months (day 111) and 6 months (day 202) post second immunization. Cells were stimulated in vitro with E7 ME peptide and analyzed via ELISpot for IFN-γ secretion. Two-sided hypothesis test for comparisons using one-way ANOVA model; ns, not significant; **, p < 0.01; ***, p < 0.001