Fig. 3

LZTFL1 is a target gene of miR-21. a Screening for miR-21 target genes by prediction softwares MIRDB, TargetScan, PicTar, and miRanda. b The overall survival rates of breast cancer patients with low (n = 319) or high (n = 307) expression levels of LZTFL1 were estimated with the Kaplan–Meier method by log-rank test according to data from the Cancer Genome Atlas (TCGA). c The predicted binding site of miR-21 in the 3′-UTR of wild type and mutant LZTFL1. d and e HEK-293 T cells were co-transfected with pRL-TK carrying a wild-type or mutant 3′-UTR sequence of LZTFL1 and the miR-21 precursor (60 ng) or the miR-21 inhibitor (10 pmol), and the luciferase activity was measured at 48 h. Experiments were performed in triplicate. f The protein level of LZTFL1 in Hs578T cells after transfection with miR-21 inhibitor