Fig. 1
Expression of miR-182 in healthy colon mucosa and a panel of CRC cell lines. a The CRC cell lines were investigated by qRT-PCR for miR-182 expression levels compared to a pool of normal colon mucosa samples. All cell lines showed high levels of miR-182, and in particular in the tumorigenic variant MICOL-14tum compared to MICOL14h-tert. Colon N, pool of normal colon mucosa. nRQ, normalized Relative Quantity. Mean values ± SD of 3 consecutive experiments are shown. *p < 0.01. b mir-182 inhibition in MICOL-14h-tert and MICOL-14tum cells. The evaluation of miR-182 expression levels was performed by qRT-PCR at 24, 48, and 72 h after the treatment. Data analysis was performed by ΔΔCt method, and the control groups (NT and anti-miR-NC treated cells) were used as a sample reference at each time point. Data were expressed as mean value ± SD of 3 independent experiments. nRQ, normalized Relative Quantity. *p < 0.01