Table 3 Resolving on discrepance between the 18 HPV MALDI-TOF MS and the Roche Cobas 4800 assays by sequencing
HPV genotype | Disagreement of both assays | Results of sequencing and genotyping on dis-concordant results of both assays | Conc. rate (%) | Results of sequencing and genotyping on dis-concordance | ||
|---|---|---|---|---|---|---|
Conc. | Dis-conc. | Total | ||||
HPV16 | MALDI-TOF MS+/Cobas- | 16 | 6 | 22 | 72.7 | 6 (1), 11 (1), 52 (1), 54 (1), 81 (2), 82 (1), neg(2) |
MALDI-TOF MS−/Cobas+ | 12 | 7 | 19 | 63.2 | 6 (2), 40 (1), 42 (1), 43 (2), 52 (2), 58 (1), neg(2) | |
HPV18 | MALDI-TOF MS+/Cobas- | 2 | 1 | 3 | 66.7 | neg(1) |
MALDI-TOF MS−/Cobas+ | 0 | 1 | 1 | 0 | 58 (1) | |
Other 12 HR HPV | MALDI-TOF MS+/Cobas- | 20 | 12 | 32 | 62.5 | 6 (2), 40 (2), 53 (7), 54 (1), 73 (1), 81 (2), neg(3) |
MALDI-TOF MS−/Cobas+ | 16 | 14 | 30 | 53.3 | 6 (2), 11 (2), 16 (4), 40 (1), 42 (2), 43 (1) 44 (1), 53 (2), 71 (1), 72 (1), 81 (3), 82 (2), neg(4) | |