Fig. 3

miR-328-3p and miR-370-3p directly bind to and inhibit GRM4 expression. a. The effect of 17 miRNAs on GRM4 expression was evaluated by qPCR. **P < 0.01 and ***P < 0.001. b. Western blot analysis of the effect of 17 miRNAs on GRM4 expression. c. Dual luciferase reporter gene assay showing the Firefly/Renilla luciferase activity after transfection of pGL6-pGRM4-luc, pRLTK and 17 pENTER-miRNA plasmids. d. The effect of miR-328-3p mutation on the luciferase activity suppressed by wild type miR-328-3p. e. The effect of miR-370-3p mutation on the luciferase activity suppressed by wild type miR-328-3p. f Western blot showing the effects of miR-328-3p mimic and miR-328-3p inhibitor on GRM4 expression in MCF7 and MDA-MB-231 cells. g Western blot showing the effects of miR-370-3p mimic and miR-370-3p inhibitor on GRM4 expression in MCF7 and MDA-MB-231 cells