Fig. 2

Reduction of AMOG expression affects L1-mediated apoptosis and senescence in correlation with signal transduction molecules in glioma U-87 MG cells. U-87 MG cells were transfected with either Control siRNA or AMOG siRNA. Western blot analysis for determining the levels of AMOG and L1, and L1 downstream signaling pathway proteins. Expression of AMOG and L1 (a), Akt1 and Erk1/2 phosphorylation levels (b, c), as well as the ratio of Bcl-2/Bax (d) in U-87 MG cells in response to either Control siRNA or AMOG siRNA (10 nM). Values represent means ± SEM. (* p < 0.05 and ** p < 0.01 versus Control siRNA, two-tailed paired independent Student’s t-test, n = 4). e-f Since U-87 MG cells tend to form clusters before becoming confluent X-Gal staining in the single cell state and in clusters are shown after treatment with AMOG siRNA or Control siRNA. OD values show X-Gal staining. Representative photographs of single cells (e) and cell clusters (f) are shown for X-Gal staining. Scale bars = 50 and 200 μm, respectively. g Quantification of X-Gal staining of U-87 MG cells. OD values are expressed as means ± SEM. (*p < 0.05, **p < 0.01, and ***p < 0.001, versus Control siRNA, two-tailed paired independent Student’s t-test, triplicates of three independent experiments)