Fig. 5
Hepatic activation of FOXO3 induces oxidative damage and Akt activation. a Expression of Bcl2l11 (Bim), Sesn2 and Sesn3 by qPCR in livers from 9-week-old control and FOXO3CAHep transgenic mice (n = 4). b Representative IHC staining for 8-OHdG (bar = 100 μm) and quantification of 8-OHdG-positive cells as a percentage of total hepatocyte cells in livers of patients in area of small cells (SC) and large cells (LC). c Representative IHC staining for phospho-γH2AX (bar = 100 μm) and quantification of phospho-γH2AX-positive cells as a percentage of total hepatocyte cells in livers of patients in area of small cells and large cells. d Expression of Msh2 by qPCR (upper panel), Western blot analysis for MSH2 (middle panel) and densitometric quantification (lower panel) in livers from 9-week-old control and FOXO3CAHep transgenic mice (n = 4). e Western blot analysis in livers from 9-week-old control and FOXO3CAHep mice for serine-473 phospho-Akt, with Akt and GAPDH as loading control (left panel), as well as densitometric quantification (right panel). f Representative IHC staining for phospho-Akt in livers from 9-week-old control and FOXO3CAHep mice (bar = 100 μm). g Western blot analysis in livers from 9-week-old control and FOXO3CAHep mice for Rictor and GAPDH as loading control (left panel), as well as densitometric quantification (right panel)