Fig. 5

Mast cell-released tryptase induces HUVECs proliferation and migration. a CCK-8 cell proliferation assays were performed to detect HUVECs proliferation after co-culturing for 24–72 h with mast cell supernatant or control reagents (SCF and blank). *P < 0.05. b and c HUVECs were added to the lower transwell chamber, while the upper chamber contained 20 μl of supernatant from mast cells. Tryptase- and SCF-stimulated mast cell supernatants were used as controls. After 12–72 h, the number of cells that migrated was analyzed by taking photos and counting the area per visual field. P-values, * < 0.05, ** < 0.01