Fig. 3
From: Role of protein arginine methyltransferase 5 in group 3 (MYC-driven) Medulloblastoma
PRMT5 knockdown in MYC-driven medulloblastoma cells. MYC-amplified medulloblastoma cell lines D-341 (a) and HD-MB03 (b) were transiently transfected with PRMT5-siRNA and control scrambled siRNA (SCR) for 72 h. Following transfections, cells were subjected to cell growth analyses using MTT assay and western blotting to determine the expression levels of PRMT5 and MYC proteins. The values given below each western blot are showing the densitometric quantification of each protein expression relative to the control SCR after Actin normalization. *, p < 0.05