Fig. 7
From: CRL4 ubiquitin ligase stimulates Fanconi anemia pathway-induced single-stranded DNA-RPA signaling
MLN4924 recapitulates the effects of CUL4A/B depletion. HeLa cells were treated simultaneously with an ICL-inducing agent and MLN4924 as indicated. The cisplatin and MMC concentrations were 5 μM and 1 μM, respectively. After 24 h, cells were analyzed to determine CUL4A/B modifications, RPA2, CDT1 and CHK1, and the phosphorylation of RPA2 (pS4/8 and pS33) and CHK1 (pCHK1). Tubulin served as the loading control