Fig. 4

SIRT inhibitors limit long-term cell growth and colony formation capacity of high and low metastatic NPC cells. a 5-8F and 6-10B cells were seeded in 6-well plates and treated with the drug concentration indicated for 72 h. Then, cell culture media was removed and colony formation observed for 15 days. At the end of this time period, cells were fixed with 4% paraformaldehyde and stained with crystal violet. Representative pictures from triplicate experiments are shown. b The stain was solubilised with 33% acetic acid and absorbance at 592 nm were obtained. Best fit-curves were generated to determine the IC₅₀ of each drug in 5-8F and 6-10B cells