Fig. 4

Effect of Aurora-A knockdown on in vivo radiosensitivity of radioresistant HCC cells. a The growth of subcutaneous tumor derived from HepG2-R/shAuro and HepG2-R/shcontrol cells in BALB/c athymic nude mice. Mice were treated with 8.0 Gy irradiation at seventh day post tumor cell injection. Five mice were inoculated. b Representative features of tumors 42d after inoculation using HepG2-R/shAuro and HepG2-R/shcontrol cells treated with IR. c Western blotting was used to detect the Aurora-A protein expression in tumors developed from HepG2-R/shAuro and HepG2-R/shcontrol cells treated with IR, respectively. The internal control was GAPDH. d Immunostaining of PCNA protein expression in tumors developed from HepG2-R/shAuro and HepG2-R/shcontrol cells treated with IR. Lower: immunostaining; Upper: H&E staining; Bars, 100 μm. e TUNEL assay was used to detect the apoptosis in tumors developed from HepG2-R/shAuro and HepG2-R/shcontrol cells treated with IR, respectively. Data represent the mean ± S.E. of three individual experiments with triplicates. *p < 0.05 and**p < 0.01