DNA methylation of SFRP1, SFRP2, and WIF1 and prognosis of postoperative colorectal cancer patients

BMC Cancer

Table 4 Univariate and multivariate Cox analysis for association between methylation and OS in 307 CRC patients

Variables	Number		Univariate Cox		Multivariate Cox		Propensity score-1		Propensity score-2
Variables	Patients (N = 307)	Deaths (N = 127)	Crude HR (95% CI)	P value	Adjusted HR ^a (95% CI)	P value	Adjusted HR ^a (95% CI)	P value	Adjusted HR ^b (95% CI)	P value
SFRP1				0.001		0.070		0.084		0.096
Hypomethylation	55	31	1.000		1.000		1.000		1.000
Hypermethylation	252	96	0.505 (0.336–0.760)		0.672 (0.437–1.032)		0.686 (0.447–1.051)		0.688 (0.443–1.069)
SFRP2				0.001		0.005		0.015		0.034
Hypomethylation	264	119	1.000		1.000		1.000		1.000
Hypermethylation	43	8	0.307 (0.149–0.633)		0.343 (0.164–0.718)		0.410 (0.200–0.842)		0.455 (0.219–0.944)
WIF1				0.399		0.688		–		–
Hypomethylation	224	98	1.000		1.000		–		–
Hypermethylation	83	29	0.836 (0.552–1.267)		1.092 (0.710–1.682)		–		–
Co-methylation-2				0.001		0.003		0.013		0.030
Co-methylation-2 L ^c	264	119	1.000		1.000		1.000		1.000
Co-methylation-2H ^d	43	8	0.298 (0.145–0.612)		0.333 (0.159–0.694)		0.398 (0.192–0.821)		0.442 (0.212–0.923)
Co-methylation-3				0.018		0.032		0.077		0.155
Co-methylation-3 L ^e	284	123	1.000		1.000		1.000		1.000
Co-methylation-3H ^f	23	4	0.300 (0.110–0.814)		0.326 (0.117–0.908)		0.401 (0.146–1.106)		0.479 (0.174–1.321)

^aControlling for the variables which included age, gender, CEA, CA19–9, TNM staging, pathological classification, differentiation degree and postoperative radiotherapy
^bControlling for the variables which included age, gender, CEA, CA19–9, multiple polyps, tumor location, TNM staging, pathological classification, histologic classification, differentiation degree, postoperative chemotherapy and postoperative radiotherapy
^cCo-methylation-2 L: patients with promoter hypomethylation of at least one gene (SFRP1 or SFRP2)
^dCo-methylation-2H: patients with promoter hypermethylation of SFRP1 and SFRP2
^eCo-methylation-3 L: patients with promoter hypomethylation of at least one gene (SFRP1 or SFRP2 or WIF1)
^fCo-methylation-3H: patients with promoter hypermethylation of SFRP1, SFRP2 and WIF1

ISSN: 1471-2407