Fig. 4

LINC00689 is taken for a ceRNA to regulate USP6NL by sponging miR-142-3p in TNBC. a. RT-qPCR detected the expression of LINC00689 in TNCB cells. b-c. The cytoplasmic location of LINC00689 in TNBC cells was identified by subcellular fraction assay and FISH assay. d. The expression of USP6NL in both cells was assessed by RT-qPCR in the case of LINC00689 overexpression. e. RNA pull down assay was used to confirm the interaction between LINC00689 and miR-142-3p. f. The binding sequence between LINC00689 and miR-142-3p was acquired via ENCORI. g. The combination between LINC00689 and miR-142-3p were identified by luciferase reporter assay. h. USP6NL expression was measured by RT-qPCR under the circumstance of overexpressed LINC00689 or together with miR-142-3p. *P < 0.05, **P < 0.01