Somatic mutation detection efficiency in EGFR: a comparison between high resolution melting analysis and Sanger sequencing

Table 1 Mean C_t obtained using allele-specific probe Real time PCR

MAF (%)	MutDNA (FAM) (mean ± sd)	wtDNA (VIC) (mean ± sd)	ΔC_t (mutDNA-wtDNA) (mean ± sd)
50	24.35 ± 0.32	19.27 ± 0.39	5.08 ± 0.29
10	24.03 ± 0.56	18.55 ± 0.47	5.47 ± 0.56
5	25.18 ± 0.27	19.01 ± 0.28	6.17 ± 0.36
1	30.83 ± 0.91	20.23 ± 0.28	10.6 ± 1.01
0.1	34.26 ± 0.89	20.37 ± 0.29	13.89 ± 1.15
WT	38.33 ± 2.89	22 ± 0.01	16.33 ± 2.89

ISSN: 1471-2407