Fig. 3

Staining of Kv2.1 and Kv1.5 in inflammatory and peritumoral gastric cells. Staining of Kv2.1 (a and c) and Kv1.5 (b, d, e and f) in inflammatory (on the right) and peritumoral gastric cells (on the left), achieved using specific antibodies raised against the C-terminal of the Kv2.1 and Kv1.5 alpha subunits. a Positive staining of Kv2.1 in peritumoral cells (GX100). b Positive staining of Kv1.5 in peritumoral cells (GX100). c Nuclear localisation of Kv2.1 in inflammatory cells (GX400); d Cytoplasmic and nuclear localisation of Kv1.5 in inflammatory cells (GX400). e and f Simultaneous staining of Kv1.5 in tumour and peritumoral cells (GX100) and (GX200). Localisation of staining for Kv2.1 (g and h) and Kv1.5 (i and j) in gastric cancer cells (on the right). g Cytoplasmic and nuclear co-localisation of Kv2.1 (GX200); h Cytoplasmic localisation of Kv2.1 (GX200); i Cytoplasmic localisation of Kv1.5 protein (GX400); j Cytoplasmic and membranous co-localisation of Kv1.5 (GX200)