Fig. 5
Immunohistochemical analysis of YTS-1 xenograft tumors. a-f Individual tumor tissue sections from the following groups: (a) no treatment (cold control); (b) IF7C(10BPA)RR injection (cold IF7C(10BPA)RR); (c) IF7K(10BSH)RR injection (cold IF7K(10BSH)RR); (d) Neutron irradiation (hot control); (e) IF7C(10BPA)RR-mediated BNCT (hot IF7C(10BPA)RR); and (f) IF7K(10BSH)RR-mediated BNCT (hot IF7K(10BSH)RR). Tissues were stained with HE (left column) or indicated antibodies. Yellow square indicated enlarged images. Yellow arrows indicate CD8α-positive lymphocytes. g The number of CD8+ lymphocytes infiltrating a given tumor area (n/mm2) in indicated groups. Results are expressed as means ± SD. *P < 0.05 (Kruskal–Wallis test). h The number of CD31+ blood vessels in a given tumor area (n/mm2) of indicated groups. Results are expressed as means ± SD. N.S.: no significant difference (Kruskal–Wallis test). (i) Ki-67 proliferation index of indicated groups. Results are expressed as means ± SD. *P < 0.05 (Kruskal–Wallis test)