Fig. 4

Identification of UBE2Q1 as a poor prognostic and predictive marker for high grade serous ovarian carcinoma. a Kaplan Meier survival plots correlate high expression of UBE2Q1 (Probe id = 222480_at) with reduced relapse free survival probability of ovarian cancer patients with hazard ratio of 1.33 (b) Increased expression of UBE2Q1 was observed in tumor samples of high grade serous ovarian cancer patients (n = 11) compared to non-malignant fallopian tube epithelium tissues (n = 24) using validation dataset GSE10971. The UBE2Q1 levels in experimental dataset GSE66957 is also plotted for comparison. c Western blot analysis depicts increase in levels of UBE2Q1 in OVCAR8 high grade serous ovarian cancer cells compared to A2780 cells. Total Histone H3 was used as loading control. The uncropped Western blot images are provided in supplementary Fig. 3. The densitometric analysis of Western blots was performed using ImageJ. d The serous ovarian cancer patient cohorts (grade III) were treated with combination of platin/taxane and the response outcomes were determined by relapse free survival over 12 months. UBE2Q1 expression was significantly higher in non-responders (n = 65) to platin/taxane drug treatment when compared to responders (n = 126); p-value was 0.00051 as calculated by Mann-Whitney test. e ROC plot illustrates the predictive biomarker value of UBE2Q1 in response to platin/taxane therapy with AUC value equals to 0.654 (p value = 1.2e-04)