Fig. 2

KLF5 is a positive regulator of Eppk1 in Hela cell. a, b HeLa cells were infected with adenoviruses expressing green fluorescent protein (Ad-GFP) or KLF5 (Ad-KLF5) for 24 h (10 ng/mL). KLF5 and Eppk1 protein and mRNA expression were analyzed by Western blot (a) and qRT-PCR (b), respectively. *P < 0.05 and **P < 0.01 vs. the Ad-GFP. c, d HeLa cells were transfected with non-specific short interfering RNA (si-Con) or KLF5-specific siRNA (si-KLF5) for 24 h. KLF5 and Eppk1 protein and mRNA expression was analysed by western blot (c) and qRT-PCR (d), respectively. *P < 0.05 vs. the si-Con