Fig. 3

miR-222 inhibitor reduces the CRC tumorigenesis and immune escape induced by MSC-EVs. a, tumor volume in xenograft models; b, assessment of tumorigenesis by tumor weight; c, percentage of CD3⁺ cells in tumor tissues detected by immunohistochemistry; d, miR-222 expression in cells by in situ hybridization; e, expression of immune escape-related proteins in tumor tissues examined by western blot. Full-length blots are presented in Additional file 3 (Supplementary Fig. S2). Measurement data were presented as mean ± SD. Unpaired t test was applied to analyze the differences between two experimental groups (panel b and c), while two-way ANOVA to analyze data among multiple groups, along with Tukey’s post hoc test (panel a and e)