Fig. 7
From: CD36 promotes vasculogenic mimicry in melanoma by mediating adhesion to the extracellular matrix
Integrin-α3 supports melanoma cell adhesion to laminin. (A) Flow cytometric analysis of integrin-α3 expression on C32 melanoma cells with isotype control (dotted line) and integrin-α3 (solid line). (B) Adhesion of C32 melanoma cells on to laminin coated coverslips for 60 min prior to staining for integrin-α3 (left panel), paxillin (middle panel) and a nuclear stain (DAPI, colored merged image). Dual stained focal contacts shown in high magnification insert (bottom right). Isotype control stains shown in the top insert. Scale bar = 10 μm. (C) Adhesion of C32 melanoma cells treated with an anti-integrin-α3 antibody or isotype control onto tissue culture plates coated with 50 μg/ml laminin. Microscopy images (left) illustrate the density of adherent cells on each well surface. Scale bar = 500 μm. Bar graph (right), quantification of C32 melanoma cell adhesion to laminin as assessed following treatment with a blocking anti-integrin-α3 antibody or isotype control. Results are normalized to control (ctl) wells and are mean ± SEM from n = 3 experiments. *p < 0.05, t-test