Fig. 2

Estrogen reduces circulating neutrophil in tumour-bearing mice. Experiment is conducted according to the description in Fig. 1. A, Tumour burden increases circulating neutrophils which were reduced with estrogen treatment in both Inv and Null mice; Percentage of circulating neutrophils (CD45⁺CD11b⁺Ly6G⁺) out of viable CD45⁺ population via flow cytometry analysis, Null+Ctrl (no tumour - N.T) n = 3, Null+Ctrl n = 8, Null+E2V n = 8, Inv + Ctrl (N.T) n = 6, Inv + Ctrl n = 12, Inv + E2V n = 13. B, Number of neutrophils per ml of blood, Null+Ctrl (N.T) n = 3, Null+Ctrl n = 5, Null+E2V n = 5, Inv + Ctrl n = 5, Inv + E2V n = 6. C, Estrogen-induced reduction of circulating neutrophils in tumour-bearing mice were not due to increase in other circulating leukocytes; (i) Percentage of circulating monocytes (CD45⁺CD11b⁺Ly6C^hi) out of viable CD45⁺ population, (ii) Percentage of circulating CD4 T-cells (CD45⁺CD4⁺CD8⁻) out of viable CD45⁺ population, (iii) Percentage of circulating CD8 T-cells (CD45⁺CD8⁺CD4⁻) out of viable CD45⁺ population via flow cytometry analysis, Null+Ctrl (N.T) n = 3, Null+Ctrl n = 8, Null+E2V n = 8, Inv + Ctrl (N.T) n = 6, Inv + Ctrl n = 12, Inv + E2V n = 13. Data represented as mean ± SEM. Statistical significances determined using one-way ANOVA followed by post-hoc tukey test