Fig. 2

Effect of TQ on TNBC cell methylation status. A Dot blot by anti-5-methylcytosine (5-mC) antibody with and without TQ treatment indicating DNA amount. B Sonicated genomic DNA. Lane “Marker” indicates DNA molecular weight size (bp). C Dual peak model results. D For methylation status, ChIP-Seq was performed using anti-MBD1 antibody, which identified > 1000 segments of the genome, of which the methylation level was affected by TQ. These segments were spanned through 136 genes. We verified the sequence laid to 14 kb and immediately upstream of IL17RD by PCR. Immediate upstream region was set as a control (Ctrl) for ChIP verification. Agarose gel electrophoresis of PCR products indicated that the methylation level of these sequences was inhibited by TQ treatment. E Quantitative analysis results from Fig. 1D. The ChIP results of immediate upstream as a control were used to normalize to ChIP results of IL17RD promoter region