Fig. 3

Effect of beta-(1→3)(1→6)-glucan from yeast on the proliferation of the breast cancer Multicellular Tumor Spheroids (MCTS) (3D). The figure shows the percent variation of proliferation of T47D spheroids with different β-glucan concentrations (0.1–1 − 10 and 100 µg/ml) after 24 h, 48 h, and 72 h incubation compared with untreated spheroids (control). The proliferation was measured after 4 h incubation of untreated- and with β-glucan treated breast cancer spheroids using XTT by 450 nm. The results were presented in three independent experiments. Statistical significance was determined by two-way ANOVA. * p < 0.05; ** p < 0.01; *** p < 0.001; **** p < 0.0001