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Fig. 2 | BMC Cancer

Fig. 2

From: MMP-11 expression in early luminal breast cancer: associations with clinical, MRI, pathological characteristics, and disease-free survival

Fig. 2

Characterization of the specificity of the anti MMP-11 antibody. Western blot analysis of different MMPs expression using the anti-MMP-11 antibody (5ST-4A9) in whole cell protein extracts (20 µg) of transfected HEK293 cells. Cells were either non-transfected (lane 1) or transfected with vectors encoding, MMP-2 (lane2), MMP-9 (lane 3), MMP-11 (lane 4) and MMP-14 (lane 5). The anti-MMP-11 specific antibody recognized a single protein of around 55KDa in MMP-11 transfected cells. Anti-MMP-2, MMP-9 and MMP-14 antibodies were utilized as controls and the anti-Rab7 antibody was used as a loading control

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