Fig. 1

EGR1 expression in human pancreatic cancer cell lines. (A) Expression of EGR1 in pancreatic cancer and normal pancreatic tissues from The Cancer Genome Atlas (TCGA) and Genotype-Tissue Expression (GTEx) databases. (B-D) qRT-PCR and Western blot analysis showed higher expression of EGR1 in four pancreatic cancer cell lines (AsPC-1, BxPC-3, CFPAC-1, PANC-1) than in HPDE cells. Samples were from the same experiment and gels/blots were processed in parallel. (E-F) PANC-1 and CFPAC-1 cells were treated with different concentrations (0.01, 0.1, 1 µmol/L) of Gemcitabine for 48 h.(G-H) PANC-1 and CFPAC-1 cells were treated with 0.1 µmol/L concentration of Gemcitabine for 24, 48 and 72 h. The qRT-PCR analysis was performed to determine the expression levels of EGR1 mRNA. Data are expressed as mean ± SD. *P < 0.05; **P < 0.01; ***P < 0.001