Fig. 2

EGR1 mediates MDR1 transcriptional activity regulating gemcitabine resistance. (A) OE-EGR1 and OE-NC cells were treated with 0.1 µmol/L gemcitabine for 48 h. Changes in MDR1 and EGR1 were detected by Western blot analysis.(B) The sh-EGR1 cells and sh-NC cells were treated with 0.1 µmol/L gemcitabine for 48 h. Changes in MDR1 and EGR1 were detected by Western blot analysis. (C-D)Binding of EGR1 to the MDR1 promoter was detected using the ChIP assay. (E-F) Baseline expression of MDR1 in pancreatic cancer cell lines was detected by qRT-PCR and Western blot analysis.(G-H)Expression of MDR1 after gemcitabine treatment of CFPAC-1 and PANC-1 cells as detected by qRT-PCR.Samples were from the same experiment and gels/blots were processed in parallel.Data are expressed as mean ± SD. *P < 0.05; **P < 0.01; ***P < 0.001