Table 4 Correlation between different IHC and methylation markers

Ki67 (%)

  ≤ 10% (low)

(n = 13)

7 (61.5)

6 (46.2)

10 (76.9)

3 (23.1)

0 (0.0)

 10% ~ 60% (medium)

(n = 42)

35 (83.3)

16 (38.1)

24 (57.1)

13 (31.0)

4 (9.5)

  ≥ 60% (high)

(n = 33)

31 (90.9)

18 (54.5)

6 (18.2)

10 (30.3)

17 (51.5)

 CHITEST

P = 0.0065

P = 0.3508

P < 0.0001

P = 0.9077

P < 0.0001

NapsinA

  + 

(n = 57)

39 (68.4)

29 (50.9)

57 (100.0)

0 (0.0)

0 (0.0)

to LUAC SENS:85.1% SPEC:100%

 -

(n = 57)

51 (89.5)

24 (42.1)

10 (17.5)

30 (52.6)

17 (29.8)

 CHITEST

P = 0.0081

P = 0.4212

P < 0.0001

P < 0.0001

P < 0.0001

TTF-1

  + 

(n = 85)

64 (75.3)

48 (56.5)

67 (78.8)

1 (1.2)

17 (20.0)

to LUAC and SCLC SENS:84.0% SPEC:97.7%

 -

(n = 59)

50 (84.7)

20 (33.9)

10 (16.9)

43 (72.9)

6 (10.2)

 CHITEST

P = 0.1261

P = 0.0098

P < 0.0001

P < 0.0001

P = 0.0422

CK5/6

  + 

(n = 57)

46 (80.7)

22 (38.6)

13 (22.8)

43 (75.4)

1 (1.8)

to LUSC SENS:95.6% SPEC:79.7%

 -

(n = 57)

45 (78.9)

28 (49.1)

39 (68.4)

2 (3.5)

16 (28.1)

 CHITEST

P = 0.8214

P = 0.3355

P < 0.0001

P < 0.0001

P < 0.0001

P63

  + 

(n = 31)

27 (87.1)

9 (29.0)

6 (19.4)

25 (80.6)

0 (0.0)

to LUSC SENS:96.2% SPEC:86.7%

 -

(n = 40)

32 (80.0)

20 (50.0)

27 (67.5)

1 (2.5)

12 (30.0)

 CHITEST

P = 0.3944

P = 0.0926

P = 0.0003

P < 0.0001

P < 0.0001

P40

  + 

(n = 36)

30 (83.3)

12 (33.3)

2 (5.5)

34 (94.4)

0 (0.0)

to LUSC SENS:97.1% SPEC:97.0%

 -

(n = 70)

54 (77.1)

40 (57.1)

50 (67.5)

5 (7.1)

15 (21.4)

 CHITEST

P = 0.2383

P = 0.0090

P < 0.0001

P < 0.0001

P < 0.0001