Fig. 4

PRKAA2 expression in malignant cells is associated with liver hepatocellular carcinoma (LIHC) development. A Clinical and molecular features of 18 LIHC patients from the Chinese National Genebank Database (CSE0000008) and the proportion of cell types in each patient. B T-distributed stochastic neighbor embedding plot of 14,236 cells colored based on cell types or PRKAA2 expression. C Uniform manifold approximation and projection plot of 2730 malignant cells colored based on cell subgroups or PRKAA2 expression. D Heatmap of copy number profile genes in malignant cells with high PRKAA2 expression and low PRKAA2 expression. E The enriched pathways in malignant cells with high PRKAA2 expression and low PRKAA2 expression using gene set variation analysis (Bayesian t-test). F Differences in PRKAA2 expression and pathway scores between primary tumors and relapsed tumors (Wilcoxon rank test). G Differences in the expression of cyclin genes between malignant cells with high PRKAA2 expression and low PRKAA2 expression (Wilcoxon rank test; *P < 0.05, **P < 0.01, ***P < 0.001)