Fig. 7

PRKAA2 is associated with dynamic change of CD4+ T cells during tumor progression. A Uniform manifold approximation and projection plot of 4813 CD4+ T cells colored based on cell types or PRKAA2 expression. B The proportion of five types of CD4+ T cells expressing high or low levels of PRKAA2. C The levels of immune checkpoint proteins, including CD27, TIGIT, TNFRSF9, ICOS, TNFRSF4, CTLA4, TNFRSF18, and CD28 in the five types of CD4+ T cells. D The proportion of CD4+ Treg cells expressing high or low levels of PRKAA2 (Wilcoxon rank test). (E-G) Differentiation trajectory of CD4+ T cells colored for cell types E, PRKAA2 expression F, and pseudotime G. (H-I) The significantly enriched metabolic pathways in Treg cells H and T cells with high PRKAA2 expression I were identified by gene set enrichment analysis