Fig. 11

Effect of NUP155 silencing on TNBC cell lines MDA-MB-231 and BT-549. A-B RT-PCR validation of NUP155 silencing efficiency. Western blot analysis to verify NUP155 silencing efficiency in MDA-MB-231 cells (C-D) and BT-549 cells (E-F). G Wound healing assay to analyze the impact of NUP155 silencing on TNBC cell healing ability. H-I CCK8 assay to analyze the effects of NUP155 silencing on the proliferation of MDA-MB-231 and BT-549 cells. Western blot analysis of the decrease in the BCL2/BAX expression ratio in MDA-MB-231 cells (J-K) and BT-549 cells (L-M) due to NUP155 siRNA. N Transwell assay to analyze the impact of NUP155 silencing on cell migration. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001. The blots were cut prior to hybridisation with antibodies during blotting, and the three replicates of original blots of Fig. 11C, E and J, and Fig. 11L are presented in the Supplementary material