Fig. 4

Isobologram of cell viability after drug combination treatment. Cells were treated with (A) AUY922 and SAHA, (B) INK128 and SAHA, or (C) INK128 and panobinostat, for 3 days and cell viability was measured by CCK8 luminescent assay. Relative growth value (left) was determined by comparing their growth with DMSO-treated cells or blank media. Mean values of relative growth were shown as a heatmap (n = 4). EOBs (right) were then calculated as previously and their values were also shown as a heatmap. Positive EOBs indicate synergism and negative EOBs indicate antagonism