Fig. 5

LDA plots using phenotypic profiles of KS statistics after drug combination treatment. The HC analysis extracted cellular features from images of cells treated with different drug compounds at multiple doses. Phenotypic profiles of each drug treatment were calculated by KS statistics and analyzed by LDA. Two independent experiments (A and B) were carried out to test different combinations of drugs. The first data set (A) included all possible combinations of HSP90 inhibitors, HDAC inhibitors, DNA inhibitors, and proteasome inhibitors. The second data set (B) included all possible combination of the previous drugs together with mTOR inhibitor or MT inhibitors. Two different timepoints such as 24-h and 48-h treatment were analyzed separately and their distinct phenotypic profiles were shown by the first two discriminant components. Single treatments were distinguished by different colors and combinations by gray color. Different drug concentrations were also distinguished by different sizes, and different timepoints by different transparencies. The single drug treatments with the second highest drug concentration (C2), which were used as the training data set, were highlighted in solid colors