Fig. 2
From: Identification of BACH1-IT2-miR-4786-Siglec-15 immune suppressive axis in bladder cancer
BACH1-IT2 upregulated Siglec-15 in bladder cancer cells. A Comparison of BACH1-IT2 abundance in Siglec-15 negative and positive bladder tumors. B Representative images of Siglec-15 immunohistochemistry (IHC) staining results. C Correlation between endogenous BACH1-IT2 and Siglec-15expression in bladder tumors. D Establishment of BACH1-IT2-knockdown cell lines in UMUC-3 and T24 cells was validated by real-time PCR (upper pane) and according Siglec-15 was quantified (lower pane). E Establishment of BACH1-IT2-overexpressing cell lines in HT1197 and SW780 cells was validated by real-time PCR and according Siglec-15 was quantified (lower pane). F Western blot analysis of Siglec-15 protein in BACH1-IT2 knockdown or overexpressing cells, and the croppted blots were presented correspondingly. G Immunofluorescence staining of cell surface Siglec-15 of HT1197 in response to either control (NC) or BACH1-IT2 overexpression