Fig. 3
From: Identification of BACH1-IT2-miR-4786-Siglec-15 immune suppressive axis in bladder cancer
BACH1-IT2-upregulated Siglec-15 was dependent on miR-4786. A Quantitative analysis of Siglec-15 transcript in BACH1-IT2 intact or depleted UMUC-3 and T24 cells treated with control or miR-4786 inhibitor. Endogenous miR-4786 was quantified accordingly (upper left pane). B Quantitative analysis of Siglec-15 transcript in BACH1-IT2 naïve or overexpressing HT1197 and SW780 cells treated with control or miR-4786 mimics. Endogenous miR-4786 was quantified accordingly (upper left pane). C Western blot results of Siglec-15 protein in the cells as described in A and B, and the croppted blots were presented correspondingly. D Immunofluorescence staining of cell surface Siglec-15 in HT1197 cells expressing either naïve or ectopic BACH1-IT2, and transfected with either control or miR-4786 mimics