Fig. 5

BACH1-IT2 functioned as ceRNA against miR-4786 in bladder cancer. A Relative expression of miR-4786 in both UMUC-3 and T24 cells transfected with either negative control or BACH1-IT2-specific shRNA. B Relative expression of miR-4786 in both HT1197 and SW780 cells transfected with either control or BACH1-IT2-expressing plasmids. C RNA-pulldown results in both UMUC-3 and T24 cells with control, wild-type LINC0973 fragment, or corresponding mutant, respectively. D Relative expression of BACH1-IT2 in both HT1197 and SW780 cells transfected with either negative control or miR-4786 inhibitors. E Relative expression of BACH1-IT2 in both UMUC-3 and T24 cells transfected with either control or miR-4786 mimics. F RNA-pulldown results in both HT1197 and SW780 cells with either control or miR-4786. G Reverse correlation analysis between endogenous miR-4786 and BACH1-IT2 in bladder tumor samples (n = 100). H Illustration of aligned miR-4786 with both wild-type and mutated BACH1-IT2. I Luciferase reporter results in 293 T cells co–transfected with BACH1-IT2 (wt and mut) and miR-NC, miR-4786 mimic, and miR-4786 inhibitor, respectively