Fig. 6

BACH1-IT2-miR-4786-Siglec-15 axis involved in immune activations in bladder cancer cells. A Establishment of Siglec-15 knockdown cells in UMUC-3 and T24 was confirmed by real-time PCR. B Establishment of Siglec-15 overexpression cells in HT1197 and SW780 cells was confirmed by real-time PCR. C Western blot results of relative expression of Siglec-15 protein in cells described in A and B, and the croppted blots were presented correspondingly. D Interleukin-2 (IL-2) production analysis in Jurkat: UMUC-3/T24 (shNT or shSiglec-15, 2:1) co–culture system. IL-2 was quantified with an ELISA kit at 48 and 72 h, respectively. E IL-2 production analysis in Jurkat: HT1197/SW780 (EV or Siglec-15, 2:1) co–culture system. F IL-2 production analysis of Jurkat co–culture system with both HT1197 and SW780 cells transfected with control or BACH1-IT2 in combination with either miR-NC or miR4786 mimics. G IL-2 production analysis of Jurkat co–culture system with both UMUC-3 and T24 cells transfected with negative control or shBACH1-IT2 in combination with either miR-NC or miR-4786 inhibitor. H Tumor necrosis factor-α (TNF-α) secretion analysis of Jurkat co–culture system with both UMUC-3 and T24 cells transfected with negative control or shBACH1-IT2 in combination with either miR-NC or miR-4786 inhibitors. I TNF-α secretion analysis of Jurkat co–culture system with both HT1197 and SW780 cells transfected with either control or BACH1-IT2 in combination with either miR-NC or miR-4786 mimics