Fig. 5

RTEL1 activated JNK signaling pathway and ELK1 in glioma cells. (A) ROS production was determined by flow cytometry. Knockdown of RTEL1 significantly promoted ROS release but abolished by ROS scavenger NAC. Mann–Whitney U test was used and data were expressed as mean ± SD. *** p < 0.001 represents si-N.C v.s si-RTEL1; ^###p < 0.001 represents si- RTEL1 v.s si-RTEL1 + NAC. (B) Western blot analysis of RTEL1 Knockdown combination with NAC treatment in U87 and SF295 cells with GAPDH as the normalized controls. (C) Western blot analysis of RTEL1 overexpression combination with AS602801 treatment in SF295 cells with GAPDH as the normalized controls. si-N.C represents siRNA of non-target as controls; si-RTEL1-1724 represents siRNA of RTEL1; NAC represents ROS scavenger N-acetyl-L-cysteine; AS602801 represents JNK inhibitor (10 μM)