Fig. 4

Overexpressing c-myc reversed the decreased cell viability, colony number, migration and invasion cell numbers caused by silencing SIRT5 in U-CH1 and U-CH2 cells. The expression of c-myc after c-myc overexpression in U-CH1 and U-CH2 cells was detected by A, RT-qPCR and B, Western blot; C, CCK-8 assay was performed to assess the viability of U-CH1 and U-CH2 cells in each group; D, Cell colonies were evaluated by colony formation analysis(scale bars = 0.5 cm); E, The colony number in each group in U-CH1 and U-CH2 cells; F, Transwell assay was performed to detect cell migration (magnification, ×200); G, The migration cell number in each group in U-CH1 and U-CH2 cells; H, Cell invasion was detected by Transwell assay (magnification, ×200); I, The invasion cell number in each group in U-CH1 and U-CH2 cells. SIRT, sirtuin; RT-qPCR, reverse transcription-polymerase chain reaction; CCK-8, cell counting kit-8; sh-RNA, short hairpin RNA.